ITS2 secondary structure data improves authentication of Moringa oleifera tea products when using with DNA barcoding

نویسندگان

چکیده

Adulteration is a severe issue affecting the herbal industry. Therefore, robust tool needed to address this problem. In current study, Moringabased products (tea) authentication was conducted using DNA barcoding. Two different barcode regions, rbcL and ITS2, were investigated in terms of their effectiveness authenticating products. To proceed with barcoding, good quality gDNA prerequisite for PCR amplification. Hence, two lysis buffers, PL1 PL2, compared obtain gDNA. Results revealed that higher yield obtained from fresh plants but lower tea except sample P3. The reaction successfully by amplifying barcodes, ITS2. amplicon size plant 643 bp 404 contrast, generated sizes 672 P1, 679 P2, P3, 674 P4. For ITS2 395 406 398 413 amplified analyzed bioinformatic tools. neighbour-joining (NJ) analysis indicated P4 categorized same clade M. oleifera sequence GenBank. Simultaneously, P1 clustered individually other closely related species. showed all samples grouped clade. Incorporating secondary structure prediction after NJ improved discrimination between

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ژورنال

عنوان ژورنال: Plant science today

سال: 2023

ISSN: ['2348-1900']

DOI: https://doi.org/10.14719/pst.2094